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quick block immunostaining blocking solution p0260  (Beyotime)


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    Beyotime quick block immunostaining blocking solution p0260
    Quick Block Immunostaining Blocking Solution P0260, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 6583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunostaining+blocking+solution/Crystal+Violet+Staining+Solution/pm41075604-206-9-70
    Average 99 stars, based on 6583 article reviews
    quick block immunostaining blocking solution p0260 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1.
    Article Snippet: This study investigates the role of advanced glycation end-products (AGEs) in tumor vasculogenic mimicry (VM).. Using high-sugar diet animal models and glycated extracellular matrix (ECM) ex vivo models, AGEs derived is demonstrated from glycated ECM significantly enhanced tumor growth and VM formation.. However, carbon dots (egCDs) derived from glycated ECM effectively inhibit tumor growth and VM formation in this glycated microenvironment.

    Article Title: Melittin inhibits MPP + -induced HT22 cell death by suppressing Bax activation and improving mitochondrial function
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min and permeabilised with 0.1% Triton X-100 for 30 min. After being washed with PBS and blocked with immunostaining blocking solution (Beyotime Biotechnology), cells were incubated with anti-AIF antibody (1:200, CST, #5318) overnight at 4°C and then incubated with YF ® 594 goat anti-rabbit IgG (1:200, UElandy) for 2 h, followed by incubation with DAPI (UElandy) for 15 min. .. Finally, cell images were acquired using a confocal laser scanning microscope (Leica).

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis
    Article Snippet: Various treatment conditions were applied for a duration of 48 h. Post-treatment, the cells were gently rinsed twice with PBS, followed by fixation using Beyotime (China) for 10 min. .. Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China). .. Following washing with PBS, the Cytochrome c oxidase IV (COX IV, CST, USA) -specific primary antibody was applied and incubated overnight.

    Article Title: Veillonella parvula outer membrane vesicles increase ICAM-1 + neutrophils exhibiting elevated NET formation via ROS–PAD4 signaling
    Article Snippet: .. Neutrophils with or without V. parvula OMVw stimulation for 4 h were fixed, permeabilized for 15 min, and blocked using an immunostaining blocking solution (Beyotime). .. Subsequently, the neutrophils were stained with the anti-rabbit ICAM-1 antibody and the anti-goat MPO antibody (R&D Systems, Minneapolis, MN, USA) at 4°C overnight.

    Article Title: Exosomes derived from MSCs exposed to hypoxic and inflammatory environments slow intervertebral disc degeneration by alleviating the senescence of nucleus pulposus cells through epigenetic modifications
    Article Snippet: .. Slides containing NPCs were initially treated with 4 % paraformaldehyde at room temperature for 30 min. Then, the cells were permeabilized with 0.3 % Triton X-100 (Solarbio, China) for 15 min and blocked with immunostaining blocking solution (Beyotime, China) for 1 h. The cells were incubated with an anti-p65 antibody (1:1000, 8242T, CST, USA) overnight at 4 °C. .. The next day, the slides of NPCs were incubated with the corresponding Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H + L) (1:500, Beyotime, China) in the dark at room temperature for 1 h. Then, the slides were stained with DAPI (Beyotime, China) in the dark at room temperature for 10 min.

    Article Title: Crocin promotes ferroptosis in gastric cancer via the Nrf2/GGTLC2 pathway
    Article Snippet: Following 24 h of crocin treatment (where applicable), cells were fixed with 1 mL of immunostaining fixative (Beyotime, P0098) for 10 min at room temperature and washed twice with immunostaining wash solution (Beyotime, P0106). .. Blocking was performed using immunostaining blocking solution (Beyotime, P0102) for 60 min at room temperature. .. Primary antibodies, anti-Ki-67 (Abcam, ab16667) and anti-Nrf2 (Abcam, ab137550), were diluted 1:250 in immunostaining primary antibody dilution solution (Beyotime, P0103) and incubated at 4°C for 12 h. The secondary antibody, goat anti-rabbit IgG H&L (Alexa Fluor ® 647) (Abcam, ab150079), was diluted 1:500 in immunofluorescence secondary antibody dilution solution (Beyotime, P0108) and incubated at room temperature in the dark for 60 min.

    Article Title: Endothelial MICU1 protects against vascular inflammation and atherosclerosis by inhibiting mitochondrial calcium uptake
    Article Snippet: .. Immunofluorescence staining and confocal microscopy For immunofluorescence staining of sections of thoracic aorta or aortic sinus, the sections were permeabilized with 0.1% Triton X-100 at room temperature for approximately 15 min. Then, the sections were blocked with the immunostaining blocking solution (Beyotime, China, Cat# P0102) at room temperature for 1 h. After the blocking solution was discarded, the primary antibodies of VCAM1 (Abcam, Cat# ab134047), SIRT3 (Cell Signaling Technology, Cat# 5490), SOD2 (Cell Signaling Technology, Cat# 5490) and Ac-SOD2 (Abcam, Cat# ab137037) were added and placed at 4 °C overnight. ..

    Article Title: Protective effects of AER-271 in acute-phase radiation-induced brain injury in rats: reduction of brain edema, inflammation, apoptosis and maintenance of blood-brain barrier integrity
    Article Snippet: .. Antigen repair was performed using sodium citrate antigen repair solution (Beyotime, Jiangsu, China) in a water bath at 100°C for 20 min, and the tissues were closed with immunostaining blocking solution (Beyotime, Jiangsu, China) at 37°C for 30 min, followed by overnight incubation at 4°C with the following primary antibodies. .. After washing with PBS, the tissues were incubated with Alexa-Fluor 488 and 555 donkey anti-rabbit/mouse secondary antibodies (Beyotime, Jiangsu, China) for 2 h at room temperature.

    Blocking Assay:

    Article Title: Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1.
    Article Snippet: This study investigates the role of advanced glycation end-products (AGEs) in tumor vasculogenic mimicry (VM).. Using high-sugar diet animal models and glycated extracellular matrix (ECM) ex vivo models, AGEs derived is demonstrated from glycated ECM significantly enhanced tumor growth and VM formation.. However, carbon dots (egCDs) derived from glycated ECM effectively inhibit tumor growth and VM formation in this glycated microenvironment.

    Article Title: Melittin inhibits MPP + -induced HT22 cell death by suppressing Bax activation and improving mitochondrial function
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min and permeabilised with 0.1% Triton X-100 for 30 min. After being washed with PBS and blocked with immunostaining blocking solution (Beyotime Biotechnology), cells were incubated with anti-AIF antibody (1:200, CST, #5318) overnight at 4°C and then incubated with YF ® 594 goat anti-rabbit IgG (1:200, UElandy) for 2 h, followed by incubation with DAPI (UElandy) for 15 min. .. Finally, cell images were acquired using a confocal laser scanning microscope (Leica).

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis
    Article Snippet: Various treatment conditions were applied for a duration of 48 h. Post-treatment, the cells were gently rinsed twice with PBS, followed by fixation using Beyotime (China) for 10 min. .. Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China). .. Following washing with PBS, the Cytochrome c oxidase IV (COX IV, CST, USA) -specific primary antibody was applied and incubated overnight.

    Article Title: Veillonella parvula outer membrane vesicles increase ICAM-1 + neutrophils exhibiting elevated NET formation via ROS–PAD4 signaling
    Article Snippet: .. Neutrophils with or without V. parvula OMVw stimulation for 4 h were fixed, permeabilized for 15 min, and blocked using an immunostaining blocking solution (Beyotime). .. Subsequently, the neutrophils were stained with the anti-rabbit ICAM-1 antibody and the anti-goat MPO antibody (R&D Systems, Minneapolis, MN, USA) at 4°C overnight.

    Article Title: Exosomes derived from MSCs exposed to hypoxic and inflammatory environments slow intervertebral disc degeneration by alleviating the senescence of nucleus pulposus cells through epigenetic modifications
    Article Snippet: .. Slides containing NPCs were initially treated with 4 % paraformaldehyde at room temperature for 30 min. Then, the cells were permeabilized with 0.3 % Triton X-100 (Solarbio, China) for 15 min and blocked with immunostaining blocking solution (Beyotime, China) for 1 h. The cells were incubated with an anti-p65 antibody (1:1000, 8242T, CST, USA) overnight at 4 °C. .. The next day, the slides of NPCs were incubated with the corresponding Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H + L) (1:500, Beyotime, China) in the dark at room temperature for 1 h. Then, the slides were stained with DAPI (Beyotime, China) in the dark at room temperature for 10 min.

    Article Title: Crocin promotes ferroptosis in gastric cancer via the Nrf2/GGTLC2 pathway
    Article Snippet: Following 24 h of crocin treatment (where applicable), cells were fixed with 1 mL of immunostaining fixative (Beyotime, P0098) for 10 min at room temperature and washed twice with immunostaining wash solution (Beyotime, P0106). .. Blocking was performed using immunostaining blocking solution (Beyotime, P0102) for 60 min at room temperature. .. Primary antibodies, anti-Ki-67 (Abcam, ab16667) and anti-Nrf2 (Abcam, ab137550), were diluted 1:250 in immunostaining primary antibody dilution solution (Beyotime, P0103) and incubated at 4°C for 12 h. The secondary antibody, goat anti-rabbit IgG H&L (Alexa Fluor ® 647) (Abcam, ab150079), was diluted 1:500 in immunofluorescence secondary antibody dilution solution (Beyotime, P0108) and incubated at room temperature in the dark for 60 min.

    Article Title: Endothelial MICU1 protects against vascular inflammation and atherosclerosis by inhibiting mitochondrial calcium uptake
    Article Snippet: .. Immunofluorescence staining and confocal microscopy For immunofluorescence staining of sections of thoracic aorta or aortic sinus, the sections were permeabilized with 0.1% Triton X-100 at room temperature for approximately 15 min. Then, the sections were blocked with the immunostaining blocking solution (Beyotime, China, Cat# P0102) at room temperature for 1 h. After the blocking solution was discarded, the primary antibodies of VCAM1 (Abcam, Cat# ab134047), SIRT3 (Cell Signaling Technology, Cat# 5490), SOD2 (Cell Signaling Technology, Cat# 5490) and Ac-SOD2 (Abcam, Cat# ab137037) were added and placed at 4 °C overnight. ..

    Article Title: Protective effects of AER-271 in acute-phase radiation-induced brain injury in rats: reduction of brain edema, inflammation, apoptosis and maintenance of blood-brain barrier integrity
    Article Snippet: .. Antigen repair was performed using sodium citrate antigen repair solution (Beyotime, Jiangsu, China) in a water bath at 100°C for 20 min, and the tissues were closed with immunostaining blocking solution (Beyotime, Jiangsu, China) at 37°C for 30 min, followed by overnight incubation at 4°C with the following primary antibodies. .. After washing with PBS, the tissues were incubated with Alexa-Fluor 488 and 555 donkey anti-rabbit/mouse secondary antibodies (Beyotime, Jiangsu, China) for 2 h at room temperature.

    Incubation:

    Article Title: Melittin inhibits MPP + -induced HT22 cell death by suppressing Bax activation and improving mitochondrial function
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min and permeabilised with 0.1% Triton X-100 for 30 min. After being washed with PBS and blocked with immunostaining blocking solution (Beyotime Biotechnology), cells were incubated with anti-AIF antibody (1:200, CST, #5318) overnight at 4°C and then incubated with YF ® 594 goat anti-rabbit IgG (1:200, UElandy) for 2 h, followed by incubation with DAPI (UElandy) for 15 min. .. Finally, cell images were acquired using a confocal laser scanning microscope (Leica).

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis
    Article Snippet: Various treatment conditions were applied for a duration of 48 h. Post-treatment, the cells were gently rinsed twice with PBS, followed by fixation using Beyotime (China) for 10 min. .. Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China). .. Following washing with PBS, the Cytochrome c oxidase IV (COX IV, CST, USA) -specific primary antibody was applied and incubated overnight.

    Article Title: Exosomes derived from MSCs exposed to hypoxic and inflammatory environments slow intervertebral disc degeneration by alleviating the senescence of nucleus pulposus cells through epigenetic modifications
    Article Snippet: .. Slides containing NPCs were initially treated with 4 % paraformaldehyde at room temperature for 30 min. Then, the cells were permeabilized with 0.3 % Triton X-100 (Solarbio, China) for 15 min and blocked with immunostaining blocking solution (Beyotime, China) for 1 h. The cells were incubated with an anti-p65 antibody (1:1000, 8242T, CST, USA) overnight at 4 °C. .. The next day, the slides of NPCs were incubated with the corresponding Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H + L) (1:500, Beyotime, China) in the dark at room temperature for 1 h. Then, the slides were stained with DAPI (Beyotime, China) in the dark at room temperature for 10 min.

    Article Title: Protective effects of AER-271 in acute-phase radiation-induced brain injury in rats: reduction of brain edema, inflammation, apoptosis and maintenance of blood-brain barrier integrity
    Article Snippet: .. Antigen repair was performed using sodium citrate antigen repair solution (Beyotime, Jiangsu, China) in a water bath at 100°C for 20 min, and the tissues were closed with immunostaining blocking solution (Beyotime, Jiangsu, China) at 37°C for 30 min, followed by overnight incubation at 4°C with the following primary antibodies. .. After washing with PBS, the tissues were incubated with Alexa-Fluor 488 and 555 donkey anti-rabbit/mouse secondary antibodies (Beyotime, Jiangsu, China) for 2 h at room temperature.

    Immunofluorescence:

    Article Title: Endothelial MICU1 protects against vascular inflammation and atherosclerosis by inhibiting mitochondrial calcium uptake
    Article Snippet: .. Immunofluorescence staining and confocal microscopy For immunofluorescence staining of sections of thoracic aorta or aortic sinus, the sections were permeabilized with 0.1% Triton X-100 at room temperature for approximately 15 min. Then, the sections were blocked with the immunostaining blocking solution (Beyotime, China, Cat# P0102) at room temperature for 1 h. After the blocking solution was discarded, the primary antibodies of VCAM1 (Abcam, Cat# ab134047), SIRT3 (Cell Signaling Technology, Cat# 5490), SOD2 (Cell Signaling Technology, Cat# 5490) and Ac-SOD2 (Abcam, Cat# ab137037) were added and placed at 4 °C overnight. ..

    Staining:

    Article Title: Endothelial MICU1 protects against vascular inflammation and atherosclerosis by inhibiting mitochondrial calcium uptake
    Article Snippet: .. Immunofluorescence staining and confocal microscopy For immunofluorescence staining of sections of thoracic aorta or aortic sinus, the sections were permeabilized with 0.1% Triton X-100 at room temperature for approximately 15 min. Then, the sections were blocked with the immunostaining blocking solution (Beyotime, China, Cat# P0102) at room temperature for 1 h. After the blocking solution was discarded, the primary antibodies of VCAM1 (Abcam, Cat# ab134047), SIRT3 (Cell Signaling Technology, Cat# 5490), SOD2 (Cell Signaling Technology, Cat# 5490) and Ac-SOD2 (Abcam, Cat# ab137037) were added and placed at 4 °C overnight. ..

    Confocal Microscopy:

    Article Title: Endothelial MICU1 protects against vascular inflammation and atherosclerosis by inhibiting mitochondrial calcium uptake
    Article Snippet: .. Immunofluorescence staining and confocal microscopy For immunofluorescence staining of sections of thoracic aorta or aortic sinus, the sections were permeabilized with 0.1% Triton X-100 at room temperature for approximately 15 min. Then, the sections were blocked with the immunostaining blocking solution (Beyotime, China, Cat# P0102) at room temperature for 1 h. After the blocking solution was discarded, the primary antibodies of VCAM1 (Abcam, Cat# ab134047), SIRT3 (Cell Signaling Technology, Cat# 5490), SOD2 (Cell Signaling Technology, Cat# 5490) and Ac-SOD2 (Abcam, Cat# ab137037) were added and placed at 4 °C overnight. ..



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    Image Search Results


    The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Fluorescence

    The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Journal: iScience

    Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

    doi: 10.1016/j.isci.2025.114190

    Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

    Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

    Techniques: Immunostaining, Staining

    The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Journal: Materials Today Bio

    Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis

    doi: 10.1016/j.mtbio.2025.102009

    Figure Lengend Snippet: The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

    Article Snippet: Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China).

    Techniques: Knockdown, Expressing